Roman Fedoryshchak

Project: Lipidated proteomics: Chemical validation of protein lipidation pathways as antifungal targets

This project profiles the substrates of protein lipidation of plant pathogentic fungi, more specifically N-myristoyltransferase (NMT). The Tate lab has developed powerful tools for analysis of this enzyme in living systems, and we will exploit these capabilities to establish in vivo mode of action for known and novel NMT inhibitors as anti-fungal agents. The technology features a strong emphasis on exploiting synthetic chemistry to understand agrochemical mode of action and enables facile incorporation into target proteins without disturbing the system of interest, whilst permitting imaging and pull-down based on a highly optimized 'chemical proteomic' workflow.